Sweeney M J, Kay C, Klotz L R, Klotz S D
Ann Allergy. 1981 Jun;46(6):295-300.
A method for the detection of specific IgE in human serum is described, using a modified radioimmune assay termed the Inhibition Assay Technique (IAT) The technique is based on the ability of specific IgE to bind with allergen. The difference in total IgE before incubation with the allergen and after incubation with the allergen gives a relative concentration of specific IgE to that particular allergenic substance. The assay has been shown to correlate with both the intradermal skin test as well as the standard RAST now used to determine specific IgE.
本文描述了一种检测人血清中特异性IgE的方法，该方法采用一种改良的放射免疫测定法，称为抑制测定技术（IAT）。该技术基于特异性IgE与变应原结合的能力。在与变应原孵育前和孵育后的总IgE差异给出了针对该特定变应原物质的特异性IgE的相对浓度。已证明该测定法与皮内皮肤试验以及目前用于测定特异性IgE的标准RAST均相关。
