Schellenberg R R, Adkinson N F
J Immunol. 1975 Dec;115(6):1577-83.
A technique for the absolute quantification of antigen-specific human IgE is described. It employs elution of a calculable amount of antigen-specific IgE from an allergosorbent-antibody complex by means of alkaline pH treatment, followed by measurement of the IgE content of the eluate with a modified radioimmunosorbent test (RIST). With this method IgE antibody directed against the benzylpenicilloyl determinant of penicillin (BPO) was measured quantitatively in sera from seven penicillin allergic patients. IgE specific for ragweed antigen E was measured in sera from 33 ragweed allergic patients. Values obtained for IgE anti-BPO ranged from 19 to 1806 ng/ml and comprised from 1.3 to 27.5% of total serum IgE. Values of IgE anti-antigen E ranged from 9 to 1807 ng/ml, comprising from 3 to 84% of total serum IgE. Excellent correlation (r = 0.99; p less than 0.001) was obtained for both antigen systems between values determined by the RAST elution technique and by simple RAST assay with interpolation from a reference serum of known specific IgE content as determined by the elution technique may be needed only for primary standardization of reference sera.
本文描述了一种用于抗原特异性人IgE绝对定量的技术。该技术通过碱性pH处理从变应原吸附剂 - 抗体复合物中洗脱可计算量的抗原特异性IgE，然后用改良的放射免疫吸附试验（RIST）测量洗脱液中的IgE含量。用这种方法对7例青霉素过敏患者血清中针对青霉素苄基青霉噻唑决定簇（BPO）的IgE抗体进行了定量测定。对33例豚草过敏患者血清中针对豚草抗原E的特异性IgE进行了测定。抗BPO IgE的值范围为19至1806 ng/ml，占血清总IgE的1.3%至27.5%。抗抗原E IgE的值范围为9至1807 ng/ml，占血清总IgE的3%至84%。对于两种抗原系统，通过RAST洗脱技术测定的值与通过简单RAST测定并根据洗脱技术测定的已知特异性IgE含量的参考血清进行插值得到的值之间具有极好的相关性（r = 0.99；p小于0.001），可能仅需对参考血清进行一级标准化时才需要用洗脱技术测定。
