Ferreira P C, Paucker M, Golgher R R, Paucker K
Arch Virol. 1981;68(1):27-33. doi: 10.1007/BF01315164.
The chromatographic behavior of human amniotic interferon on various affinity chromatography ligands was studied. Most of this interferon bound strongly to bovine plasma albumin-agarose, cibacron blue F3GA-agarose, concanavalin A-agarose and L-tryptophyl-L-tyrosine-omega-carboxyl-pentyl-agarose. After binding most of the interferon activity was eluted only with 50 percent ethylene glycol, showing the high hydrophobicity of this interferon. Smaller quantities could be recovered after phosphate-buffered saline elution or with increased salt concentration. On BPA-omega-carboxy-pentyl-agarose and omega-amino-hexyl-agarose, the majority of the biological activity was found in the break-through fraction (eluted with phosphate buffered saline) while some interferon was displaced with high salt or ethylene glycol. Increasing the salt concentration and lowering the pH was necessary to elute interferon from zinc chelate-agarose. These patterns indicate that human amniotic interferon is similar to human fibroblast (beta) interferon but different from human leukocyte (alpha) interferon. However, the heterogeneity displayed by amniotic interferon on bovine plasma albumin-agarose requires further investigation.
研究了人羊膜干扰素在各种亲和色谱配体上的色谱行为。这种干扰素的大部分与牛血浆白蛋白 - 琼脂糖、汽巴克隆蓝F3GA - 琼脂糖、伴刀豆球蛋白A - 琼脂糖和L - 色氨酰 - L - 酪氨酸 - ω - 羧基 - 戊基 - 琼脂糖强烈结合。结合后，大部分干扰素活性仅用50%的乙二醇洗脱，表明这种干扰素具有高度疏水性。用磷酸盐缓冲盐水洗脱或增加盐浓度后可回收较少量。在BPA - ω - 羧基 - 戊基 - 琼脂糖和ω - 氨基 - 己基 - 琼脂糖上，大部分生物活性存在于穿透组分中（用磷酸盐缓冲盐水洗脱），而一些干扰素会被高盐或乙二醇置换。从锌螯合琼脂糖上洗脱干扰素需要增加盐浓度并降低pH值。这些模式表明人羊膜干扰素与人类成纤维细胞（β）干扰素相似，但与人类白细胞（α）干扰素不同。然而，羊膜干扰素在牛血浆白蛋白 - 琼脂糖上表现出的异质性需要进一步研究。
