Duc-Goiran P, Robert-Galliot B, Chudzio T, Chany C
Proc Natl Acad Sci U S A. 1983 May;80(9):2628-31. doi: 10.1073/pnas.80.9.2628.
Interferon (IFN) induced in the human amniotic membrane contains at least five different molecular species, as shown by analysis in NaDodSO4/polyacrylamide gels after heating and under reducing conditions. Three of the IFN components reported here--migrating at 26, 43, and 80 kilodaltons--are of unusual antigenic structure because they are neutralized to about the same extent by anti-IFN-alpha and anti-IFN-beta antibodies. The 15- to 17-kilodalton species belongs to the IFN-alpha group, while the 21- to 22-kilodalton species, the most frequently detected major peak, is IFN-beta. In addition to their unusual size and antigenic structure, these IFNs could play a role during embryonic development and in the immune tolerance of the mother with regard to the fetus.
如在加热及还原条件下于十二烷基硫酸钠/聚丙烯酰胺凝胶中分析所示，人羊膜中诱导产生的干扰素（IFN）至少包含五种不同的分子种类。本文报道的三种干扰素成分——分子量分别为26、43和80千道尔顿——具有不同寻常的抗原结构，因为它们被抗IFN-α和抗IFN-β抗体中和的程度大致相同。分子量为15至17千道尔顿的种类属于IFN-α组，而分子量为21至22千道尔顿的种类是最常检测到的主峰，为IFN-β。除了其不同寻常的大小和抗原结构外，这些干扰素可能在胚胎发育过程中以及母体对胎儿的免疫耐受方面发挥作用。
