Berger E M, Cox G, Weber L, Kenney J S
Biochem Genet. 1981 Apr;19(3-4):321-31. doi: 10.1007/BF00504277.
In a permanent cell line derived from Drosophila embryos, cytoplasmic actin is produced as an unstable precursor, which is subsequently converted to a stable form. This conversion results in a reduction in isoelectric point, with no apparent change in molecular weight. The conversion involves an enzymatic acetylation, and results in an insensitivity to aminopeptidase digestion, suggesting N-terminal blockage. Both the acetylated and unacetylated actins can participate in the assembly of F-actin, but with different efficiencies.
在源自果蝇胚胎的永久性细胞系中，细胞质肌动蛋白以不稳定的前体形式产生，随后转化为稳定形式。这种转化导致等电点降低，而分子量没有明显变化。该转化涉及酶促乙酰化，并导致对氨肽酶消化不敏感，表明N端被封闭。乙酰化和未乙酰化的肌动蛋白都可以参与F-肌动蛋白的组装，但效率不同。
