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无细胞系统中合成蛋白质的N端乙酰化的预防。

Prevention of NH2-terminal acetylation of proteins synthesized in cell-free systems.

作者信息

Palmiter R D

出版信息

J Biol Chem. 1977 Dec 25;252(24):8781-3.

PMID:925022
Abstract

The NH2 terminus of ovalbumin is acetylated in cell-free protein-synthesizing systems as it is in vivo. The acetyl group is derived from acetyl-CoA and it is incorporated during translation. Acetylation can be prevented by metabolizing the available acetyl-CoA to citrate with the addition of citrate synthase and oxalacetate to the translation system. The NH2 terminus of ovalbumin synthesized under these conditions can be sequenced by automated Edman degradation. This procedure has also been applied to the sequencing of Pr 76gag, the viral core protein precursor synthesized from 35 S Rous sarcoma virus RNA.

摘要

在无细胞蛋白质合成系统中，卵清蛋白的氨基末端如同在体内一样被乙酰化。乙酰基来源于乙酰辅酶A，且在翻译过程中被掺入。通过向翻译系统中添加柠檬酸合酶和草酰乙酸，将可用的乙酰辅酶A代谢为柠檬酸，可防止乙酰化。在这些条件下合成的卵清蛋白的氨基末端可通过自动化学降解法进行测序。该方法也已应用于Pr 76gag的测序，Pr 76gag是由35S劳氏肉瘤病毒RNA合成的病毒核心蛋白前体。

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Prevention of NH2-terminal acetylation of proteins synthesized in cell-free systems.无细胞系统中合成蛋白质的N端乙酰化的预防。
J Biol Chem. 1977 Dec 25;252(24):8781-3.
2
Ovalbumin: a secreted protein without a transient hydrophobic leader sequence.卵清蛋白：一种无瞬时疏水前导序列的分泌蛋白。
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FEMS Microbiol Lett. 2000 Mar 1;184(1):35-40. doi: 10.1111/j.1574-6968.2000.tb08986.x.

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查尔酮合酶 mRNA 的编码和 3'非编码核苷酸序列以及酶的氨基酸序列的分配。
EMBO J. 1983;2(10):1801-5. doi: 10.1002/j.1460-2075.1983.tb01661.x.
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