Barsumian E L, Isersky C, Petrino M G, Siraganian R P
Eur J Immunol. 1981 Apr;11(4):317-23. doi: 10.1002/eji.1830110410.
The rat basophilic leukemia (RBL) cell lines were cloned and the various sublines compared for their chromosome number, IgE-mediated histamine release and for IgE surface receptors. It was found that cell lines started from tumors at different times vary in both their chromosome number and their ability to release histamine by an IgE-mediated reaction. RBL-I and III have approximately 44 chromosomes and did not respond to an IgE-mediated reaction. RBL-II and RBL-IV have 68-73 chromosomes and showed moderate levels of histamine release (percent release mean = 5 +/- 2 and 10 +/- 4, respectively). The cloning of the RBL-IV line resulted in some sublines which were excellent histamine releasers (range 39-100%) and some which were relatively refractory (less than 10%) to IgE-mediated histamine release. These clones did not differ significantly in chromosome number. Recloning the releasing lines gave rise to poor releasers, whereas the recloning of poor releasers did not produce good releasers indicating that the mutational drift in culture is toward loss of histamine-releasing capacity. The number of IgE receptors and the rate of IgE association and dissociation were similar for the different cell lines. The study failed to disclose significant molecular weight differences in the IgE receptor from the various clones and sublines indicating that the failure to release probably does not reside in the receptor. The various cloned sublines are phenotypically stable, and the isolation of excellent histamine-releasing sublines are useful for studies of the complex phenomenon of the histamine release.
对大鼠嗜碱性白血病（RBL）细胞系进行克隆，并比较各个亚系的染色体数目、IgE介导的组胺释放以及IgE表面受体情况。结果发现，不同时间从肿瘤起始的细胞系在染色体数目以及通过IgE介导反应释放组胺的能力方面均存在差异。RBL-I和III大约有44条染色体，对IgE介导的反应无应答。RBL-II和RBL-IV有68 - 73条染色体，组胺释放水平中等（释放百分比平均值分别为5±2和10±4）。RBL-IV细胞系的克隆产生了一些组胺释放良好的亚系（范围为百分之39 - 100%）和一些对IgE介导的组胺释放相对不敏感的亚系（低于10%）。这些克隆在染色体数目上没有显著差异。对释放能力强的细胞系再次克隆会产生释放能力弱的细胞系，而对释放能力弱的细胞系再次克隆则不会产生释放能力强的细胞系，这表明培养过程中的突变漂移是朝着组胺释放能力丧失的方向发展。不同细胞系的IgE受体数量以及IgE结合和解离速率相似。该研究未能揭示不同克隆和亚系的IgE受体在分子量上存在显著差异，这表明释放失败可能并不在于受体。各个克隆亚系在表型上是稳定的，分离出组胺释放良好的亚系对于研究组胺释放这一复杂现象很有用处。
