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作为特异性免疫吸附剂的抗原脉冲巨噬细胞单层：对可溶性蛋白抗原有特异性的小鼠T细胞的选择性吸收

Antigen-pulsed macrophage monolayers as specific immunoabsorbents: selective absorption of murine T cells committed to soluble protein antigen.

作者信息

Langdon W Y, Holt P G, Shellam G R

出版信息

Immunology. 1981 Jul;43(3):555-62.

PMID:6166549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1555067/
Abstract

Antigen-pulsed macrophage (MO) monolayers in petri dishes were successfully employed as immunoabsorbents, for both the depletion and enrichment of T cells which proliferated upon exposure to soluble ovalbumin (OA). This procedure was shown not to deplete T cells reactive to a second antigen, and similarly MO monolayers pulsed with other antigens failed to deplete the T-cell preparation of OA-reactive cells. Employing brief incubation with trypsin/ethylenediamine tetracetic acid (EDTA), up to 2 X 10(6) OA-binding T cells could be recovered from each petri dish.

摘要

培养皿中的抗原脉冲巨噬细胞（MO）单层已成功用作免疫吸附剂，用于消耗和富集暴露于可溶性卵清蛋白（OA）后增殖的T细胞。该方法显示不会消耗对第二种抗原反应的T细胞，同样，用其他抗原脉冲的MO单层也无法消耗OA反应性细胞的T细胞制剂。通过与胰蛋白酶/乙二胺四乙酸（EDTA）短暂孵育，每个培养皿中可回收多达2×10⁶个结合OA的T细胞。

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本文引用的文献

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Immunology. 1981 Jan;42(1):137-47.
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The regulatory effect of macrophages on the antigen-induced lymph node cell proliferative response.
Cell Immunol. 1980 Feb;49(2):317-28. doi: 10.1016/0008-8749(80)90033-7.
3
Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells.抗原包被柱上的细胞分离。去除高速率抗体形成细胞和免疫记忆细胞。
J Exp Med. 1969 Jan 1;129(1):23-36. doi: 10.1084/jem.129.1.23.
4
Receptors on immunocompetent cells. I. Receptor specificity of cells participating in a cellular immune response.免疫活性细胞上的受体。I. 参与细胞免疫应答的细胞的受体特异性。
Cell Immunol. 1970 Oct;1(4):404-18. doi: 10.1016/0008-8749(70)90017-1.
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Lymphocyte proliferation in vitro induced by hapten autologous protein conjugates. I. A study on the class of lymphocytes responding in vitro and on the nature and specificity of their receptors.半抗原自体蛋白缀合物诱导的体外淋巴细胞增殖。I. 体外应答淋巴细胞类别及其受体性质和特异性的研究
J Exp Med. 1974 Mar 1;139(3):732-53. doi: 10.1084/jem.139.3.732.
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A rapid method for the isolation of functional thymus-derived murine lymphocytes.一种分离功能性胸腺来源的小鼠淋巴细胞的快速方法。
Eur J Immunol. 1973 Oct;3(10):645-9. doi: 10.1002/eji.1830031011.
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A new, simple method for the preparation of lymphocytes bearing specific receptors.一种制备带有特异性受体淋巴细胞的全新简易方法。
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