雏鸡颈二腹肌的组织化学特性：多重神经支配与慢张力纤维类型之间的关系

Histochemical properties of the biventer cervicis muscle of the chick: a relationship between multiple innervation and slow-tonic fibre types.

作者信息

Toutant J P, Rouaud T, Le Douarin G H

出版信息

Histochem J. 1981 May;13(3):481-93. doi: 10.1007/BF01005063.

Abstract

Chick biventer cervicis muscle fibres have been studied histochemically. Fast-twitch, focally innervated (alpha) fibres represent 70-80% of the total fibres in this muscles. Two histochemical profiles of slow-tonic multi-innervated (beta) fibres have been observed from embryonic life the adult (three-months) stage. These two slow-tonic types differ in the activity of their histochemically demonstrated myofibrillar ATPase after either acid or alkaline preincubation, and after formalin fixation. Both slow-tonic fibre types have a high oxidative metabolism and are PAS-negative. They are referred as to beta 1 and beta 2R fibre types (slow-tonic oxidative) in an expansion of Ashmore's nomenclature, and compared to avian slow-tonic sub-types that have been described in recent reports. beta 1 and beta 2 fibre types exhibit a similar pattern of innervation. Possible explanations of the origin of histochemical heterogeneity in multiple innervated fibres are discussed.

摘要

对鸡颈二腹肌肌纤维进行了组织化学研究。快肌纤维，即局部受神经支配的（α）纤维，占该肌肉总纤维的70 - 80%。从胚胎期到成年（三个月）阶段，观察到了慢张力多神经支配（β）纤维的两种组织化学特征。这两种慢张力纤维类型在酸性或碱性预孵育后以及福尔马林固定后，其组织化学显示的肌原纤维ATP酶活性有所不同。两种慢张力纤维类型都具有高氧化代谢且过碘酸雪夫反应阴性。在扩展阿什莫尔命名法的基础上，它们被称为β1和β2R纤维类型（慢张力氧化型），并与近期报道中描述的鸟类慢张力亚型进行了比较。β₁和β₂纤维类型表现出相似的神经支配模式。文中讨论了多神经支配纤维中组织化学异质性起源的可能解释。