Shiho O, Nakagawa Y, Tsuchiya K
J Antibiot (Tokyo). 1981 Jan;34(1):79-83. doi: 10.7164/antibiotics.34.79.
Sprague Dawley (SD) rats were immunized with various penicillin-ovalbumin (OvA) in combination with aluminum hydroxide (alum) and thimerosal-killed Bordetella pertussis for the purpose of obtaining rat anti-penicillin IgE sera. In the rat 60-hour passive cutaneous anaphylaxis (PCA) reaction and the hapten inhibition test, a weak cross reaction between penicillin G (PCG) and ampicillin (ABPC) was observed, but not cross reaction was observed between sulbenicillin (SBPC) and other penicillins. Rat anti-6-formamidopenicillanic acid (FPC) IgE serum reacted with PCG-bovine gamma globulin (BGG), ABPC-BGG and SBPC-BGG, but FPC-BGG did not react with rat anti-PCG, anti-ABPC and anti-SBPR IgE sera and the PCA reaction between anti-FPC IgE sera and FPC-BGG was inhibited by FPC, PCG, ABPC and SBPC. These results indicate that the antigenic active sites of PCG, ABPC and SBPC are limited to the acyl side chain moiety of penicillins, while the antigenic active site of FPC is confined to the penicilloyl moiety of the penicillin.
为获得大鼠抗青霉素IgE血清，将不同的青霉素 - 卵清蛋白（OvA）与氢氧化铝（明矾）和硫柳汞灭活的百日咳博德特氏菌联合免疫斯普拉格 - 道利（SD）大鼠。在大鼠60小时被动皮肤过敏反应（PCA）和半抗原抑制试验中，观察到青霉素G（PCG）和氨苄西林（ABPC）之间有微弱的交叉反应，但未观察到磺苄西林（SBPC）与其他青霉素之间的交叉反应。大鼠抗6 - 甲酰胺青霉烷酸（FPC）IgE血清与PCG - 牛γ球蛋白（BGG）、ABPC - BGG和SBPC - BGG发生反应，但FPC - BGG不与大鼠抗PCG、抗ABPC和抗SBPR IgE血清发生反应，并且抗FPC IgE血清与FPC - BGG之间的PCA反应被FPC、PCG、ABPC和SBPC抑制。这些结果表明，PCG、ABPC和SBPC的抗原活性位点仅限于青霉素的酰基侧链部分，而FPC的抗原活性位点局限于青霉素的青霉噻唑基部分。
