β-内酰胺抗生素制剂的抗原性：针对β-内酰胺抗生素的IgE抗体产生及其在抗生素组内的交叉反应。

Antigenicity of beta-lactam antibiotic preparations: production of IgE antibodies to beta-lactam antibiotic and their cross-reaction within the antibiotic group.

作者信息

Iwata M, Katsuta M, Tanii T, Tokiwa H, Matuhasi T

出版信息

Int Arch Allergy Appl Immunol. 1982;68(1):35-40. doi: 10.1159/000233064.

Abstract

BALB/C mice were immunized with conjugate of benzylpenicillin or ampicillin with Ascaris suum extract. The mice developed IgE antibodies to penicillin, which were found to react with commercially available penicillin preparations in the PCA system. Elimination of polymerized penicillin by Sephadex chromatography from tested preparations could not diminish their activities to elicit PCA. High pressure liquid chromatographic (HPLC) separation of PcG preparation yielded fractions unable to elicit PCA. On the other hand, all fractions from ABPC retained the activity even after the HPLC purification. A comparative estimation of cross-reactivity of IgE and IgE antipenicillin antibodies showed that IgE antibodies cross-reacted with a variety of beta-lactam antibiotics in a high degree.

摘要

用苄青霉素或氨苄青霉素与猪蛔虫提取物的结合物对BALB/C小鼠进行免疫。小鼠产生了针对青霉素的IgE抗体，这些抗体在PCA系统中能与市售青霉素制剂发生反应。通过葡聚糖凝胶色谱法从测试制剂中去除聚合青霉素并不能降低它们引发PCA的活性。对青霉素G（PcG）制剂进行高压液相色谱（HPLC）分离得到的组分无法引发PCA。另一方面，氨苄青霉素（ABPC）的所有组分即使经过HPLC纯化后仍保留活性。对IgE和抗青霉素IgE抗体交叉反应性的比较评估表明，IgE抗体与多种β-内酰胺抗生素高度交叉反应。