Geisow M J, D'Arcy Hart P, Young M R
J Cell Biol. 1981 Jun;89(3):645-52. doi: 10.1083/jcb.89.3.645.
Intravascular pH was measured within the lysosomes and newly formed phagosomes in cultured mouse peritoneal macrophages. The kinetics of pH change in both vacuolar systems was quantitatively determined within a large cell population by fluorescence spectroscopy. Additionally, pH changes within individual phagosomes were followed semiquantitatively using indicator dyes. Two novel findings were made. Firstly, the pH in new phagosomes was transiently driven alkaline (higher than physiological) even when the external medium was buffered at pH 6.5. Secondly, perturbations of phagosome-lysosome fusion had little effect upon phagosomal pH changes, even though the compounds used markedly altered the pH of the lysosomes in resting and phagocytosing cells.
在培养的小鼠腹腔巨噬细胞的溶酶体和新形成的吞噬体中测量血管内pH值。通过荧光光谱法在大量细胞群体中定量测定两个液泡系统中pH变化的动力学。此外，使用指示剂染料半定量跟踪单个吞噬体内的pH变化。有两个新发现。首先，即使外部介质在pH 6.5下缓冲，新吞噬体中的pH值也会短暂地变为碱性（高于生理值）。其次，吞噬体 - 溶酶体融合的扰动对吞噬体pH变化影响很小，尽管所使用的化合物显著改变了静息和吞噬细胞中溶酶体的pH值。
