Fuchs O, Ponka P, Borova J, Neuwirt J, Travnicek M
J Supramol Struct Cell Biochem. 1981;15(1):73-81. doi: 10.1002/jsscb.1981.380150108.
Synthesis of globin mRNA in erythroid spleen cells from anemic mice was measured after in vitro incubation under conditions in which the level of intracellular heme was manipulated. This newly synthesized globin mRNA was isolated by hybridization with globin cDNA covalently bound to cellulose. Isonicotinic acid hydrazide (INH) and penicillamine were used as specific inhibitors of heme synthesis. It has been found that a 120-min incubation of spleen erythroid cells with 5mM INH or 5mM penicillamine reduced [3H] uridine incorporation into globin mRNA by 24% or 36%, respectively. The addition of heme to INH- or penicillamine-treated cells almost completely restored [3H] uridine incorporation into globin mRNA. These results indicate that heme stimulates transcription of processing of globin mRNA.
在体外培养条件下，通过控制细胞内血红素水平，对贫血小鼠红系脾细胞中珠蛋白mRNA的合成进行了测定。新合成的珠蛋白mRNA通过与共价结合在纤维素上的珠蛋白cDNA杂交来分离。异烟肼（INH）和青霉胺用作血红素合成的特异性抑制剂。已发现，脾红系细胞与5mM异烟肼或5mM青霉胺孵育120分钟，分别使[3H]尿苷掺入珠蛋白mRNA的量减少了24%或36%。向经异烟肼或青霉胺处理的细胞中添加血红素，几乎完全恢复了[3H]尿苷掺入珠蛋白mRNA的量。这些结果表明，血红素刺激珠蛋白mRNA的转录加工。
