Horst A, Rakowicz-Szulczyńska E M, Wiland E
Mol Cell Biochem. 1981 Jun 9;37(1):3-12. doi: 10.1007/BF02355882.
Qualitative and quantitative changes of non-histone chromatin proteins of spleen cells during the primary immune response to sheep red blood cells and aggregated human gamma-globulin were described. Synthesis of non-histone chromatin proteins was measured by labelling with 3H-tryptophan during culture of spleen cells in vitro. Chromatin was isolated and labelled proteins were analysed by polyacrylamide gel electrophoresis. During the immune response to both antigens in chromatin of spleen cells three new fractions of non-histone chromatin proteins were synthesized: fractions F1 - M = 12 000 and H - M = 3 000, specific for sheep red blood cells and fractions I1 - M less than 3 000 and B - M = 120 000, specific for human gamma-globulin. The third antigen-non-specific fraction was synthesized at the time when the primary immune reaction was finishing. These new fractions were synthesized only in one of the analysed subpopulations of spleen cells. In thymocytes (non-fully-differentiated lymphoid cells) all these fractions were absent. Changes of non-histone chromatin proteins in thymocytes during the immune response were similar to those found during stimulation to proliferation by phytohemagglutinin in vitro.
本文描述了脾细胞在对绵羊红细胞和人γ球蛋白聚合物的初次免疫反应过程中，非组蛋白染色质蛋白的定性和定量变化。通过在体外培养脾细胞时用³H-色氨酸进行标记来测定非组蛋白染色质蛋白的合成。分离染色质，并用聚丙烯酰胺凝胶电泳分析标记的蛋白质。在对脾细胞染色质中的两种抗原的免疫反应过程中，合成了三个新的非组蛋白染色质蛋白组分：分子量为12000的F1组分和分子量为3000的H组分，它们对绵羊红细胞具有特异性；分子量小于3000的I1组分和分子量为120000的B组分，它们对人γ球蛋白具有特异性。第三个抗原非特异性组分是在初次免疫反应即将结束时合成的。这些新组分仅在脾细胞的一个被分析亚群中合成。在胸腺细胞（未完全分化的淋巴细胞）中，所有这些组分均不存在。胸腺细胞在免疫反应过程中非组蛋白染色质蛋白的变化与体外受植物血凝素刺激增殖时所发现的变化相似。
