Rakowicz-Szulczyńska E M, Horst A
Mol Cell Biochem. 1981 Jun 9;37(1):13-9. doi: 10.1007/BF02355883.
Incorporation of three various amino acids ([3H]-tryptophan, [3H]-methionine or [3H]-leucine) into the non-histone chromatin proteins, synthesized in spleen cells of mice after immunization with IgG, is described. Two new fractions of non-histone chromatin proteins (I-mol. wt. below 3 000 and B1-mol. wt. about 120 000), appearing during the immune reaction were labelled with [3H]-tryptophan and [3H]-methionine but not with [3H]-leucine. Synthesis of these fractions was observed only at the time of maximal RNA synthesis. A suggestion about the role of tryptophan and methionine in non-histone chromatin proteins in the regulatory processes of gene activation is discussed on the basis of their selective binding to DNA.
本文描述了用免疫球蛋白G免疫小鼠后，脾细胞中合成的非组蛋白染色质蛋白质掺入三种不同氨基酸（[3H] - 色氨酸、[3H] - 蛋氨酸或[3H] - 亮氨酸）的情况。免疫反应过程中出现的两种新的非组蛋白染色质蛋白质组分（I - 分子量低于3000和B1 - 分子量约120000），能用[3H] - 色氨酸和[3H] - 蛋氨酸标记，但不能用[3H] - 亮氨酸标记。仅在RNA合成达到最大值时观察到这些组分的合成。基于色氨酸和蛋氨酸与DNA的选择性结合，讨论了它们在非组蛋白染色质蛋白质基因激活调控过程中的作用。
