Carrier-Malhotra L, Chaudhary K D, Murthy M R
Arch Int Physiol Biochim. 1980 Dec;88(5):485-90. doi: 10.3109/13813458009092923.
Slices of cerebral cortex and cerebellum from two-week-old rats were incubated in the presence of [14C] uridine and [methyl-3H] methionine. Incorporation of 14C- and 3H-radioactivity into 18S and 28S RNA's of the two tissues was analysed by sucrose-density-gradient centrifugation. The results show that the rates of synthesis of ribosomal RNA's as well as the pattern of methylation in the two tissues were different. Some of these differences may be ascribed to factors such as pool sizes, intracellular rate of transport of the precursors by other pathways, etc. Examination of the results indicates that some differences may consist in the actual biosynthesis and maturation of ribosomal RNA's.
将两周龄大鼠的大脑皮层和小脑切片置于含有[14C]尿苷和[甲基-3H]甲硫氨酸的环境中进行孵育。通过蔗糖密度梯度离心分析两种组织中14C和3H放射性掺入18S和28S RNA的情况。结果表明，两种组织中核糖体RNA的合成速率以及甲基化模式均不同。其中一些差异可能归因于诸如库大小、前体通过其他途径的细胞内运输速率等因素。对结果的检查表明，一些差异可能存在于核糖体RNA的实际生物合成和成熟过程中。
