Thornthwaite J T, Sugarbaker E V, Temple W J
Cytometry. 1980 Nov;1(3):229-37. doi: 10.1002/cyto.990010309.
A method for measuring DNA in tissue cells by flow cytometry utilizing a one step combination nuclear isolation-DNA fluorochrome staining procedure is described. A variety of cells and tissues, both in vivo and in vitro, was used to illustrate the universal nature of this technique. These included murine bone marrow, liver testicle, sarcoma brain tumor, rat pancreatic islets, human peripheral blood, colon mucosa, colon cancer, sarcoma and brain tumor tissues. A special nuclear isolation medium, which contained either of the DNA fluorochromes, 4',6-diamidino-2 phenylindole-2 HCl or propidium iodide, was utilized successfully to isolate single suspensions of DNA fluorochrome stained nuclei in a rapid (5-10 min), consistent manner from a variety of tissues and cells. Multiple sampling of the same tissue or comparison between whole tissues and their single cell isolates showed that a representative sample was being obtained.
描述了一种通过流式细胞术测量组织细胞中DNA的方法，该方法利用一步组合的细胞核分离-DNA荧光染料染色程序。使用了多种体内和体外的细胞和组织来说明该技术的通用性。这些包括小鼠骨髓、肝脏、睾丸、肉瘤、脑肿瘤、大鼠胰岛、人外周血、结肠黏膜、结肠癌、肉瘤和脑肿瘤组织。一种特殊的细胞核分离培养基，其中含有DNA荧光染料4',6-二脒基-2-苯基吲哚-2HCl或碘化丙啶，成功地用于以快速（5-10分钟）、一致的方式从多种组织和细胞中分离出DNA荧光染料染色的细胞核的单个悬浮液。对同一组织的多次采样或全组织与其单细胞分离物之间的比较表明，获得了代表性样本。
