Compere S J, Lively M O, MacGillivray R T
Eur J Biochem. 1981 Jun 1;116(3):437-40. doi: 10.1111/j.1432-1033.1981.tb05354.x.
Poly(A)-containing RNA was isolated from chicken liver and translated in a reticulocyte lysate protein-synthesizing system in the presence of radiolabeled amino acids. Chicken albumin was isolated from the translation products by immunoprecipitation and subjected to automated Edman radiosequencing. Comparison with the sequence of proalbumin showed that the translocation product (preproalbumin) contains an NH2-terminal extension of 18 amino acid residues. The NH2-terminal sequence of chicken preproalbumin was as follows: Met-18-Lys-Asn-Val-15-Thr-Leu-Ile-Ser-Phe-10-Ile-Phe-Leu-Phe-Ser-5-Ser-Ala-Thr- Ser-1-Arg1, where Arg1 represents the NH2-terminal residue of proalbumin. This NH2-terminal extension is very rich in hydrophobic amino acid residues and is similar to the signal sequences found in other secreted proteins. The signal sequence of chicken preproalbumin shows considerable homology with the signal sequences of rat and bovine preproalbumins, but little homology with the signal sequences of other chicken preproteins.
从鸡肝中分离出含多聚腺苷酸（Poly(A)）的RNA，并在含有放射性标记氨基酸的网织红细胞裂解物蛋白质合成系统中进行翻译。通过免疫沉淀从翻译产物中分离出鸡白蛋白，并进行自动埃德曼放射测序。与前白蛋白序列比较表明，转运产物（前原白蛋白）含有一个18个氨基酸残基的氨基末端延伸。鸡前原白蛋白的氨基末端序列如下：Met-18-Lys-Asn-Val-15-Thr-Leu-Ile-Ser-Phe-10-Ile-Phe-Leu-Phe-Ser-5-Ser-Ala-Thr-Ser-1-Arg1，其中Arg1代表前白蛋白的氨基末端残基。这个氨基末端延伸富含疏水氨基酸残基，与其他分泌蛋白中的信号序列相似。鸡前原白蛋白的信号序列与大鼠和牛前原白蛋白的信号序列有相当高的同源性，但与其他鸡前体蛋白的信号序列同源性较低。
