大鼠肝脏前白蛋白原：体外合成及部分氨基酸序列
Rat liver preproalbumin: in vitro synthesis and partial amino acid sequence.
作者信息
Strauss A W, Donohue A M, Bennett C D, Rodkey J A, Alberts A W
出版信息
Proc Natl Acad Sci U S A. 1977 Apr;74(4):1358-62. doi: 10.1073/pnas.74.4.1358.
Abstract
Rat liver poly(A)-containing RNA greatly stimulated incorporation of radioactive amino acids into protein when added to a wheat germ in vitro translation system. Approximately 7% of the labeled synthetic product was precipitated following indirect immunoprecipitation with antisera to rat serum albumin. Analysis of this material, and of the cyanogen bromide fragments derived from it, by sodium dodecyl sulfate/polyacrylamide gel electrophoresis revealed that it contained an NH2-terminal extension of about 2500 daltons when compared to rat serum albumin. Automated sequence determination of purified cell-free product labeled with various radioactive amino acids revealed the presence of 18 additional amino acids NH2-terminal to the sequence of rat proalbumin. The partial sequence of this extension was found to be: Met-X-X-X-X-Phe-Leu-Leu-Leu-Leu-Phe-X-X-X-X-X-Phe-X-proalbumin. On the basis of this evidence, the immunoprecipitable cell-free product was designated preproalbumin.
摘要
当将大鼠肝脏含聚腺苷酸（poly(A)）的RNA添加到小麦胚芽体外翻译系统中时，它极大地刺激了放射性氨基酸掺入蛋白质的过程。用抗大鼠血清白蛋白的抗血清进行间接免疫沉淀后，约7%的标记合成产物被沉淀下来。通过十二烷基硫酸钠/聚丙烯酰胺凝胶电泳对该物质及其衍生的溴化氰片段进行分析，结果显示，与大鼠血清白蛋白相比，它含有一个约2500道尔顿的氨基末端延伸。用各种放射性氨基酸标记的纯化无细胞产物的自动序列测定表明，在大鼠前清蛋白序列的氨基末端存在另外18个氨基酸。发现该延伸部分的部分序列为：甲硫氨酸-X-X-X-X-苯丙氨酸-亮氨酸-亮氨酸-亮氨酸-亮氨酸-苯丙氨酸-X-X-X-X-X-苯丙氨酸-X-前清蛋白。基于这一证据，可免疫沉淀的无细胞产物被命名为前原清蛋白。
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