Fontaine-Perus J, Chanconie M, Polak J M, Le Douarin N M
Histochemistry. 1981;71(3):313-23. doi: 10.1007/BF00495878.
The development of substance P (SP) and VIP containing structures of the quail and chick guts was studied by immunocytochemistry. The appearance of VIP and substance P nerves follows a rostrocaudal pattern from day 9 in the quail and day 10 in the chick embryo. Immunoreactive fibres are first visible in the oesophagus and at 12 days they extend over the whole length of the intestine. VIP and substance P ganglionic cells are first localized in the foregut (day 9 for VIP containing neurons and day 13 for SP ones) and observed in the mid- and hind-gut just before hatching. Transplantation on the chorioallantoic membrane (CAM) of fragments of various parts of the digestive tract were carried out to see whether in such circumstances the pattern of VIP and SP containing nerves was comparable to normal. The explants contained numerous SP and VIP immunofluorescent nerve fibres. In addition, cell bodies with VIP and SP immunoreactivity appeared brightly fluorescent in the enteric ganglia of the graft showing that these peptidergic nerve cells belong to the intrinsic innervation of the gut.
采用免疫细胞化学方法研究了鹌鹑和鸡肠道中含P物质（SP）和血管活性肠肽（VIP）结构的发育过程。从鹌鹑胚胎第9天和鸡胚胎第10天开始，VIP和P物质神经的出现呈现出从吻端到尾端的模式。免疫反应性纤维首先出现在食管，到12天时延伸至肠道全长。VIP和P物质神经节细胞首先定位于前肠（含VIP神经元为第9天，含SP神经元为第13天），并在孵化前在中肠和后肠中观察到。对消化道各部分片段进行绒毛尿囊膜（CAM）移植，以观察在这种情况下含VIP和SP神经的模式是否与正常情况相当。外植体含有大量SP和VIP免疫荧光神经纤维。此外，在移植肠神经节中，具有VIP和SP免疫反应性的细胞体发出明亮荧光，表明这些肽能神经细胞属于肠道的内在神经支配。
