Schultzberg M, Hökfelt T, Olson L, Alund M, Nilsson G, Terenius L, Elde R, Goldstein M, Said S
J Auton Nerv Syst. 1980 Mar;1(3):291-303. doi: 10.1016/0165-1838(80)90024-7.
Tissue pieces of small and large intestine from pre- and postnatal and adult rats, respectively, were transplanted to the anterior eye chamber of the rat. Immunohistochemical studies revealed high numbers of substance P-, vasoactive intestinal polypeptide (VIP)- and enkephalin-containing nerve fibers, especially in the circular smooth muscle layer and in the myenteric plexus of the intestinal tissue transplants. A small number of somatostatin-containing fibers were observed. The distribution patterns of the various peptide-containing neurons in the grafts resembled those which have been described in the intact gastro-intestinal wall. Substance P, VIP, enkephalin and somato-statin immunoreactive fibers were seen also in the host irides. No obvious differences in peptide innervation in grafts and host irides were observed between normal and sympathectomized animals and animals lesioned in the trigeminal ganglion. These findings suggest that most of the peptide-containing fibers in the intestinal transplants and the host irides originate in the transplanted tissue. Thus, it is shown that peptide neurons can survive in this transplantation model, which may be useful for functional studies of intestinal physiology including peptidergic mechanisms.
分别取自出生前、出生后及成年大鼠的小肠和大肠组织块，被移植到大鼠的前房。免疫组织化学研究显示，含有P物质、血管活性肠肽（VIP）和脑啡肽的神经纤维数量众多，尤其是在肠道组织移植物的环形平滑肌层和肌间神经丛中。观察到少量含生长抑素的纤维。移植物中各种含肽神经元的分布模式与完整胃肠壁中所描述的相似。在宿主虹膜中也可见到P物质、VIP、脑啡肽和生长抑素免疫反应性纤维。在正常动物、交感神经切除动物和三叉神经节损伤动物之间，未观察到移植物和宿主虹膜中肽能神经支配的明显差异。这些发现表明，肠道移植物和宿主虹膜中大多数含肽纤维起源于移植组织。因此，表明肽能神经元可以在这种移植模型中存活，这可能对包括肽能机制在内的肠道生理学功能研究有用。
