Yamada K, Kitamura H, Maseki Y
Histochemistry. 1981;71(4):513-20. doi: 10.1007/BF00508377.
A method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. It combines an alkaline phosphatase-labeled concanavalin A-5-bromo-3-indolyl phosphate, p-toluidine salt (Con A-ALP-BIPT) method with periodic acid-Schiff (PAS) sequence. With the present dual staining method, it is possible to color alpha-D-glucosyl and alpha-D-mannosyl residues blue and 1,2-glycol groups of neutral complex carbohydrates magenta. The validity of this method has been confirmed with appropriate histochemical controls and enzyme digestions on test tissues.
已开发出一种用于光学显微镜下中性复合碳水化合物双重染色的方法。它将碱性磷酸酶标记的伴刀豆球蛋白A-5-溴-3-吲哚基磷酸对甲苯胺盐（Con A-ALP-BIPT）方法与过碘酸希夫（PAS）序列相结合。采用目前的双重染色方法，可以将α-D-葡萄糖基和α-D-甘露糖基残基染成蓝色，将中性复合碳水化合物的1,2-二醇基团染成品红色。该方法的有效性已通过对测试组织进行适当的组织化学对照和酶消化得以证实。
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