Ueda T, Mittal A K, Fujimori O, Yamada K
Department of Anatomy, Nagoya City University Medical School, Japan.
Okajimas Folia Anat Jpn. 1994 May;71(1):51-7. doi: 10.2535/ofaj1936.71.1_51.
The high iron diamine (HID) staining procedure was combined with alcian blue pH 2.5 (AB2.5) and periodic acid-Schiff (PAS) methods for the simultaneous demonstration of carbohydrates containing sulfate esters, carboxyl groups and oxidizable vicinal diols, whereas it was sequentially employed with PAS alone to differentiate sulfate esters from oxidizable vicinal diols. A variety of rat tissues and the epidermis of four fish species were utilized to test the specificity or selectivity of these methods. The HID-AB2.5-PAS sequence largely coloured carbohydrates containing sulfate esters, carboxyl groups and oxidizable vicinal diols in brownish black, turquoise and magenta shades respectively. The HID-PAS sequence coloured sulfate esters brownish black and oxidizable vicinal diols magenta. On the basis of the experimental and control studies on the both staining techniques, these staining methods could be postulated to represent efficient and useful techniques for precise histochemical analyses and simultaneous differentiations of a variety of carbohydrates in light microscopy.
高铁二胺（HID）染色程序与pH 2.5的阿尔辛蓝（AB2.5）和过碘酸希夫（PAS）方法相结合，用于同时显示含硫酸酯、羧基和可氧化邻二醇的碳水化合物，而单独依次使用PAS可将硫酸酯与可氧化邻二醇区分开来。使用多种大鼠组织和四种鱼类的表皮来测试这些方法的特异性或选择性。HID-AB2.5-PAS序列分别将含硫酸酯、羧基和可氧化邻二醇的碳水化合物染成棕黑色、蓝绿色和品红色。HID-PAS序列将硫酸酯染成棕黑色，可氧化邻二醇染成品红色。基于对这两种染色技术的实验和对照研究，可以推测这些染色方法是用于光学显微镜下精确组织化学分析和同时区分多种碳水化合物的有效且有用的技术。
