Singal D P, Blajchman M A, Naipaul N, Joseph S
Hum Immunol. 1981 May;2(3):201-11. doi: 10.1016/0198-8859(81)90012-4.
The antigenic determinants of the combined ABO, Lewis, and Secretor genes have been detected on the surface of lymphocytes by the lymphocytotoxicity test. We have studied the role of these determinants in the primed lymphocyte test (PLT), and the data demonstrate that Lewis incompatibility causes proliferative responses in PLT. On the other hand, no effects of ABO and Secretor incompatibilities were observed in this test. The frequency of the alloantigen-reactive cells (ARC) responding to Lewis and HLA-DR antigens in PLT was estimated by the limiting dilution analysis. The frequency of ARC to allogeneic Lewis-negative donors, who are positive for the sensitizing HLA-DR antigens ranged between 1:58 and 1:97. The incidence of ARC to Lewis-positive allogeneic donors who did not carry the sensitizing HLA-DR specificity was 1:94 to 1:142. These results demonstrate the presence of lymphocyte clones that are able to respond to antigens of the Lewis system. This study suggests that non-HLA antigens belonging to the Lewis system can cause stimulation of lymphocytes in the PLT test.
通过淋巴细胞毒性试验已在淋巴细胞表面检测到ABO、Lewis和分泌型基因组合的抗原决定簇。我们研究了这些决定簇在启动淋巴细胞试验（PLT）中的作用，数据表明Lewis不相容性会导致PLT中的增殖反应。另一方面，在该试验中未观察到ABO和分泌型不相容性的影响。通过极限稀释分析估计了PLT中对Lewis和HLA-DR抗原产生反应的同种异体抗原反应性细胞（ARC）的频率。对致敏HLA-DR抗原呈阳性的同种异体Lewis阴性供体的ARC频率在1:58至1:97之间。对不携带致敏HLA-DR特异性的Lewis阳性同种异体供体的ARC发生率为1:94至1:142。这些结果证明存在能够对Lewis系统抗原产生反应的淋巴细胞克隆。这项研究表明，属于Lewis系统的非HLA抗原可在PLT试验中引起淋巴细胞刺激。
