绵羊中的一种新的血红蛋白β链变体。

A new hemoglobin beta chain variant in sheep.

作者信息

John M E, John M

出版信息

Anim Blood Groups Biochem Genet. 1977;8(4):183-90. doi: 10.1111/j.1365-2052.1977.tb01645.x.

DOI:10.1111/j.1365-2052.1977.tb01645.x

Abstract

A new hemoglobin beta chain variant referred to as Hb-E is observed in four Indian sheep breeds. Hemoglobin E is electrophoretically and chromatographically undistinguishable from hemoglobin B. However, polypeptide chain separation by urea-polyacrylamide disc gel electrophoresis and urea CM-cellulose chromatography reveals the new beta chain. The betaE chain has a slower anodic mobility than betaB chain at alkaline pH. The adult sheep hemoglobins could be classified into eight categories, namely AA, AB, AE, AC, ABC, BB, BE and EE, according to their sub-unit composition. The occurrence of betaE chain in homozygous condition or in combination with either one of the two allelic beta chains betaA or betaB, indicate that it may be an allelic variant. Gene frequencies of three hemoglobin beta chain alleles observed during the study are as follows: A = 0.192; B= 0.682; E = 0.126.

摘要

在四个印度绵羊品种中观察到一种新的血红蛋白β链变体，称为Hb-E。血红蛋白E在电泳和色谱分析中与血红蛋白B无法区分。然而，通过尿素-聚丙烯酰胺圆盘凝胶电泳和尿素CM-纤维素色谱法进行的多肽链分离揭示了新的β链。在碱性pH条件下，βE链的阳极迁移率比βB链慢。根据成年绵羊血红蛋白的亚基组成，可将其分为八类，即AA、AB、AE、AC、ABC、BB、BE和EE。βE链以纯合状态出现，或与两个等位β链βA或βB之一结合出现，这表明它可能是一个等位基因变体。研究期间观察到的三种血红蛋白β链等位基因的基因频率如下：A = 0.192；B = 0.682；E = 0.126。