Hassan A S, Milner J A
Metabolism. 1981 Aug;30(8):739-44. doi: 10.1016/0026-0495(81)90018-4.
Dietary arginine deficiency is associated with retarded growth and depressed feed intake. Nucleic acid biosynthesis as indicated by the incorporation of [6-14C]-orotic acid and [32P]-orthophosphate was significantly depressed in rats fed an arginine deficient diet. The activities of the pyrimidine enzymes, aspartate transcarbamylase (ATC) and dihydroorotate dehydrogenase (DHODH) were significantly increased in rats fed an arginine deficient diet. ATC and DHODH activities in rats fed the arginine deficient diet returned to control activities after 3 wk of feeding. Orotidine 5' phosphate decarboxylase and orotate phosphoribosyl transferase activities were not affected by dietary arginine availability. In the rat fed an arginine deficient diet there was an increase in total liver pyrimidine nucleotides and a decrease in the total purine nucleotides. Significant alterations in the individual liver nucleotides were also observed. Incubation of various tissues obtained from rats fed an arginine deficient diet or a complete diet with glutamine (5mM) revealed that the liver is the major site of orotic acid synthesis. Orotic acid production in liver slices using glutamine as the nitrogen source was significantly greater in rats fed an arginine deficient diet compared to controls. The orotic aciduria occurring in rats fed an arginine deficient diet is associated with increased synthesis and decreased utilization of pyrimidines.
膳食精氨酸缺乏与生长迟缓及采食量降低有关。用[6-¹⁴C]-乳清酸和[³²P]-正磷酸盐掺入量所表示的核酸生物合成在饲喂精氨酸缺乏日粮的大鼠中显著降低。饲喂精氨酸缺乏日粮的大鼠中嘧啶酶天冬氨酸转氨甲酰酶（ATC）和二氢乳清酸脱氢酶（DHODH）的活性显著增加。饲喂精氨酸缺乏日粮的大鼠在饲喂3周后，ATC和DHODH活性恢复到对照活性。乳清苷5'-磷酸脱羧酶和乳清酸磷酸核糖基转移酶活性不受日粮精氨酸供应情况的影响。在饲喂精氨酸缺乏日粮的大鼠中，肝脏嘧啶核苷酸总量增加，嘌呤核苷酸总量减少。还观察到肝脏中单个核苷酸有显著变化。用谷氨酰胺（5mM）对饲喂精氨酸缺乏日粮或完全日粮的大鼠的各种组织进行孵育，结果表明肝脏是乳清酸合成的主要部位。与对照组相比，以谷氨酰胺为氮源的肝脏切片中，饲喂精氨酸缺乏日粮的大鼠的乳清酸生成量显著增加。饲喂精氨酸缺乏日粮的大鼠中出现的乳清酸尿症与嘧啶合成增加和利用减少有关。
