Dutt M K
Microsc Acta. 1981 Jul;84(4):339-44.
This communication presents a new method for the preparation of azure A-SO2 for use in Feulgen procedure. The salient feature of this method lies in the fact that azure A-SO2 can be decolourised with normal hydrochloric acid and sodium thiosulphate. The pH of this dye reagent is 2.3 and it is of water colour after filtration. The pH of this dye-reagent is raised to 4.0 with an aqueous solution of sodium hydroxide. Nuclear colouration with this newly developed dye-reagent on acid-hydrolysed DNA of tissue sections becomes fairly satisfactory under the usual laboratory conditions. Staining with this dye-reagent under exposure to UV ray is, however, vastly improved within 5 minutes as compared with the control. Stained sections do withstand treatment in SO2 water without exhibiting any leaching of the dye from the nuclei. Possible mode of action of UV rays in increasing the intensity of staining as well as the speed of reaction has been suggested.
本通讯介绍了一种制备用于福尔根染色法的天青A - SO₂的新方法。该方法的显著特点在于天青A - SO₂可用普通盐酸和硫代硫酸钠进行脱色。这种染料试剂的pH值为2.3，过滤后呈水彩颜色。用氢氧化钠水溶液将该染料试剂的pH值调至4.0。在常规实验室条件下，用这种新研制的染料试剂对组织切片经酸水解的DNA进行核染色效果相当令人满意。然而，与对照相比，在紫外线照射下用这种染料试剂染色5分钟内染色效果有了极大改善。染色后的切片能够耐受在SO₂水中的处理，细胞核中的染料没有出现任何渗漏。文中还提出了紫外线增强染色强度及反应速度的可能作用方式。
