Lai J L, Deminatti M
Ann Genet. 1977 Mar;20(1):25-9.
Metaphase chromosomes and interphase nuclei have been isolated by two different procedures, using either acidic or alkaline media. The morphological integrity of the isolated material has been monitored by electron microscopy. In the acidic procedure the thickness of the chromosome fibers is reduced but the chromosome gross structure is preserved. However, electrophoretic analysis indicates complete disappearance of histones H1 and partial loss of H2A and H2B. No significant modification of acid-soluble proteins isolated from chromosomes or nuclei was observed after 5-BrdU incorporation for 72 hours.
通过两种不同的方法，分别使用酸性或碱性介质，分离出了中期染色体和间期细胞核。通过电子显微镜监测了分离材料的形态完整性。在酸性方法中，染色体纤维的厚度减小，但染色体的总体结构得以保留。然而，电泳分析表明组蛋白H1完全消失，H2A和H2B部分丢失。在掺入5-溴脱氧尿苷72小时后，未观察到从染色体或细胞核中分离出的酸溶性蛋白质有明显修饰。
