Findlay J A, Thomas N W
Acta Anat (Basel). 1980;108(4):446-62. doi: 10.1159/000145344.
The histology and cell types of the islets of Langerhans were studies in the Mongolian gerbil during post-natal development using granule stains, fluorescence methods and electron microscopy. Relative islet volume, islet size distribution, islet-duct association, islet-fat association, alpha- and D cell populations and the size distribution of secretion granules in islet cells were quantified. In adult gerbils islets have an elliptical profile enclosed by a thin collagenous capsule, a central core of beta-cells, a peripheral shell of alpha-cells and an incomplete layer of D cells between. In animals of 1 week or less, islets lack a capsule, are poorly vascularized and not innervated, though alpha, beta- and D cells are present. alpha-Cells exhibit green fluorescence during these early stages that disappears when adrenergic nerve fibres grow into the islets. Electron microscopy shows a fourth (unidentified) granular islet cell type, and small granular cells, that probably correspond to intensely fluorescent cells, were found within islets and neuroinsular complexes.
利用颗粒染色、荧光方法和电子显微镜，对蒙古沙鼠出生后发育过程中胰岛的组织学和细胞类型进行了研究。对相对胰岛体积、胰岛大小分布、胰岛-导管关联、胰岛-脂肪关联、α细胞和D细胞群体以及胰岛细胞中分泌颗粒的大小分布进行了量化。成年沙鼠的胰岛呈椭圆形，被一层薄的胶原性被膜包裹，中央为β细胞核心，外周为α细胞壳，两者之间有一层不完整的D细胞层。在1周龄及以下的动物中，胰岛没有被膜，血管化程度低且无神经支配，不过α、β和D细胞均已存在。在这些早期阶段，α细胞呈现绿色荧光，当肾上腺素能神经纤维长入胰岛时荧光消失。电子显微镜显示存在第四种（未鉴定的）颗粒状胰岛细胞类型，并且在胰岛和神经胰岛复合体中发现了可能对应于强荧光细胞的小颗粒细胞。
