Mutual connections of the raphe system and hypothalamus in relation to fever.

Previous studies in our laboratory have shown that lesions in certain brain stem regions prevent elevations of body temperature after administration of bacterial pyrogen. In the present experiments wer examined the morphology of the connections of these "brain stem thermoregulatory centers" which are represented in all raphe and paraphe nuclei of the brain stem reticular formation. The methods of anterograde degeneration and tracing of retrograde transport of horseradish peroxidase were used to identify afferent and efferent connections within this "thermoregulatory field." Abundant mutual connections between the raphe nuclei and hypothalamus were found. All nuclei of the raphe system receive afferents from the medial and lateral hypothalamus. All raphe nuclei have efferent projections to the medial reticular formation, and the raphe nuclei of the pons and mesencephalon provide ascending fibers to the hypothalamus. A lesion of any part (origin, course, termination field) of this mutual raphe-hypothalamic pathway system will prevent development of fever in response to bacterial pyrogen.