Sinowatz F, Wrobel K H
Cell Tissue Res. 1981;219(3):511-24. doi: 10.1007/BF00209990.
In the present study the development of the bovine acrosome was investigated using conventional electron-microscopical techniques as well as the phosphotungstic-acid (PTA) technique (Rambourg 1967) including enzymatic digestion experiments. As in other species and in accordance with previous light-microscopical studies (Clermont and Leblond 1955) four phases of acrosomal differentiation can be discerned; the Golgi-phase, cap-phase, acrosome-phase, and maturation-phase. In the bull no internal pattern of the acrosomal content can be observed, either with conventional uranyl acetate-lead citrate staining or with the PTA-techniques. Our results support the observation in other species (Fawcett et al. 1971) that no intrinsic polymerization or crystallization process of the acrosomal content is responsible for acrosomal shaping. Some of our results suggest the influence of external forces on acrosomal development in the bull. During the cap-phase and the acrosome-phase accumulation of smooth endoplasmic reticulum and a layer of fine filaments can be observed in the Sertoli-cell cytoplasm, immediately adjacent to the developing acrosome. A temporary influence of these structures on acrosomal development seems possible. The PTA-positive staining of the developing bovine acrosome is probably due to the presence of acrosomal glycoproteins; however, our results do not exclude the possibility that molecules other than glycoproteins contribute to the positive PTA-staining of the developing acrosome.
在本研究中，我们使用传统电子显微镜技术以及磷钨酸（PTA）技术（Rambourg，1967年），包括酶消化实验，对牛顶体的发育进行了研究。与其他物种一样，并且根据先前的光学显微镜研究（Clermont和Leblond，1955年），可以辨别出顶体分化的四个阶段：高尔基体阶段、帽状阶段、顶体阶段和成熟阶段。在公牛中，无论是使用传统的醋酸铀-柠檬酸铅染色还是PTA技术，都无法观察到顶体内容物的内部模式。我们的结果支持了在其他物种中的观察结果（Fawcett等人，1971年），即顶体内容物的内在聚合或结晶过程与顶体的形成无关。我们的一些结果表明外力对公牛顶体发育有影响。在帽状阶段和顶体阶段，可以在紧邻发育中的顶体的支持细胞细胞质中观察到滑面内质网和一层细丝的积累。这些结构对顶体发育的暂时影响似乎是可能的。发育中的牛顶体的PTA阳性染色可能是由于顶体糖蛋白的存在；然而，我们的结果并不排除除糖蛋白以外的分子对发育中的顶体的PTA阳性染色有贡献的可能性。
