Greaves M, Robinson J, Delia D, Sutherland R, Newman R, Sieff C
Ciba Found Symp. 1981;84:109-29. doi: 10.1002/9780470720660.ch7.
A library of monoclonal antibodies which show selective reactivity with particular cells or gene products (e.g. HLA-DR, glycophorin) of different cell lineages in the haemopoietic system has been compiled. Using these probes in conjunction with the fluorescence-activated cell sorter (FACS) the pattern and sequence of cell surface antigenic expression on haemopoietic progenitor cells have been mapped. A cell is identified in bone marrow which has a unique membrane phenotype and the nuclear enzyme terminal deoxynucleotidyl transferase. Its composite phenotype is identical to that seen in the common variant of acute lymphoblastic leukaemia (ALL). It is suggested that this cell is a putative B lineage progenitor which provides the major target for ALL. Detailed analysis of erythroid differentiation with monoclonal antibodies on the FACS reveals an intriguing pattern of antigenic expression in which HLA-DR, glycophorin and band III appear in sequence. HLA-DR (Ia-like antigen) may be present on all or most committed haemopoietic progenitor cells and could play an important role in cell interactions regulating early haemopoiesis.
已构建了一个单克隆抗体文库，这些抗体与造血系统中不同细胞谱系的特定细胞或基因产物（如HLA - DR、血型糖蛋白）具有选择性反应。将这些探针与荧光激活细胞分选仪（FACS）结合使用，已绘制出造血祖细胞表面抗原表达的模式和顺序。在骨髓中鉴定出一种细胞，它具有独特的膜表型和核酶末端脱氧核苷酸转移酶。其复合表型与急性淋巴细胞白血病（ALL）常见变异型中所见的表型相同。有人提出，这种细胞是一种假定的B谱系祖细胞，是ALL的主要靶细胞。在FACS上用单克隆抗体对红系分化进行的详细分析揭示了一种有趣的抗原表达模式，其中HLA - DR、血型糖蛋白和带III按顺序出现。HLA - DR（类Ia抗原）可能存在于所有或大多数定向造血祖细胞上，并可能在调节早期造血的细胞相互作用中发挥重要作用。
