Wilkin G P, Woodhams P L, Ribeiro dos Santos R
Dev Neurosci. 1981;4(4):296-306. doi: 10.1159/000112769.
Immunohistochemical studies showed that immunoglobulins from sera of patients with Chagas' disease bind specifically to endothelial cells in cryostat sections of the rodent brain. In monolayer cultures of 8-day-old rat cerebella both surface and internal labelling of the endothelial cells was detected. The contrast immune sera raised against cold-insoluble globulin (which cross-reacts with fibronectin) also labelled the surface of fibroblasts as well as the endothelial cells. It would appear that Chagasic immunoglobulins are a useful marker for endothelial cells in neural cell culture.
免疫组织化学研究表明，恰加斯病患者血清中的免疫球蛋白能特异性结合啮齿动物脑低温切片中的内皮细胞。在8日龄大鼠小脑的单层培养物中，检测到内皮细胞的表面和内部标记。针对冷不溶性球蛋白（与纤连蛋白交叉反应）产生的对照免疫血清也标记了成纤维细胞表面以及内皮细胞。看来恰加斯病免疫球蛋白是神经细胞培养中内皮细胞的一种有用标记物。
