Khoury E L, Cossio P M, Szarfman A, Marcos J C, García Morteo O, Arana R M
Am J Clin Pathol. 1978 Jan;69(1):62-5. doi: 10.1093/ajcp/69.1.62.
One hundred fifty-six of 1,250 sera from patients with presumed connective tissue and related diseases showed vascular staining on mouse liver cryostat sections when they were routinely checked for antinuclear factor by the indirect immunofluorescence test. In a third of the cases, the vascular immunofluorescent pattern was given by the EVI antibody reacting with the plasma membrane of striated muscle fibers and endothelial cells, as has been recently described to occur in Chagas' disease. This led to the detection of previously unsuspected Trypanosoma cruzi infection in 67.8% of the serum samples in which the EVI antibody was detected after observation of a positive vascular pattern with mouse liver cryostat sections. On the other hand, no significant relationship between Chagas infection and sera with other anti-striated-muscle immunofluorescent patterns that also showed a vascular staining on mouse liver cryostat sections was established. Consideration of the vascular pattern observed with the EVI antibody on mouse liver cryostat sections can be helpful in detection of previously ignored T. cruzi infection in patients who have connective-tissue diseases and related conditions. This is of interest in view of the fact that anergic immunodepressive therapy, often used in these patients, significantly alters the host-parasite relationship and may lead to severe dissemination of the parasite.
在通过间接免疫荧光试验对1250例疑似结缔组织病及相关疾病患者的血清进行抗核因子常规检测时，其中156份血清在小鼠肝脏低温切片上显示出血管染色。在三分之一的病例中，血管免疫荧光模式是由EVI抗体与横纹肌纤维和内皮细胞的质膜反应产生的，正如最近所描述的恰加斯病中的情况。在通过小鼠肝脏低温切片观察到阳性血管模式后，在检测到EVI抗体的血清样本中，67.8%检测出先前未被怀疑的克氏锥虫感染。另一方面，在恰加斯病感染与在小鼠肝脏低温切片上也显示血管染色的其他抗横纹肌免疫荧光模式的血清之间未发现显著关联。考虑在小鼠肝脏低温切片上用EVI抗体观察到的血管模式，有助于检测结缔组织病及相关病症患者中先前被忽视的克氏锥虫感染。鉴于这些患者经常使用的无反应性免疫抑制疗法会显著改变宿主与寄生虫的关系，并可能导致寄生虫的严重播散，这一点很重要。
