Kasama T, Iwata Y, Oshiro K, Uchida M, Sakaguchi Y, Namie K, Sugiura M
Diabetologia. 1981 Jul;21(1):65-9. doi: 10.1007/BF03216227.
No consensus about the antigenicity of monocomponent insulin has yet been reached. We have therefore administered different insulin preparations to rabbits and rats to determine IgG and IgE antibody production. The preparations used were porcine monocomponent insulin, conventional bovine and porcine insulin powders, porcine b-component and synthesized porcine mono-desamido-insulin and hexa-desamido-insulin. In rabbits, porcine b-component was the most antigenic preparation, followed by conventional bovine and porcine insulins. No antibody production was observed with the other preparations. In rats the 60 h passive cutaneous anaphylaxis test showed virtually no insulin IgE antibody production in response to porcine monocomponent insulin. However, if porcine b-component or porcine hexa-desamido-insulin was employed both for sensitisation and as the challenging antigen, positive skin reactions were observed with demonstration of insulin IgE antibodies. Our results confirm the low antigenicity of the pharmaceutical preparation of porcine monocomponent insulin and suggest that porcine hexa-desamido-insulin and porcine b-component administration may result in the production of reagin-type antibodies.
关于单组分胰岛素的抗原性尚未达成共识。因此，我们给兔子和大鼠注射了不同的胰岛素制剂，以测定IgG和IgE抗体的产生。所用制剂包括猪单组分胰岛素、常规牛胰岛素和猪胰岛素粉末、猪b组分以及合成的猪单去酰胺胰岛素和六去酰胺胰岛素。在兔子中，猪b组分是抗原性最强的制剂，其次是常规牛胰岛素和猪胰岛素。其他制剂未观察到抗体产生。在大鼠中，60小时被动皮肤过敏试验显示，对猪单组分胰岛素几乎没有胰岛素IgE抗体产生。然而，如果使用猪b组分或猪六去酰胺胰岛素进行致敏并作为激发抗原，则会观察到阳性皮肤反应，并证明有胰岛素IgE抗体。我们的结果证实了猪单组分胰岛素制剂的低抗原性，并表明猪六去酰胺胰岛素和猪b组分的给药可能导致产生反应素型抗体。
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