Likovský Z, Smetana K
Histochemistry. 1981;72(2):301-13. doi: 10.1007/BF00517143.
Silver staining procedure for the selective demonstration of nucleolar silver stained granules (SSG) and for the simultaneous demonstration of SSG and nucleolar silver stained matrix (SSM) were studied in smears of rat Yoshida sarcoma cells. The successful results of these procedures depend mainly on the quality of silver nitrate and formaldehyde. However, both chemicals can be easily standardized and stabilized disregarding their origin and batch. In standardized procedures (one-step procedure for the selective demonstration of SSG and two-steps procedure for the simultaneous demonstration of SSG and SSM) the silver is apparently bound to acidic groups of proteins of SSG and SSM. The proteins of SSG and SSM seem to be different but both belong to the group of acidic non-histone proteins. According to the results of digestion experiments a possibility also exists that the acidic proteins of SSG may be associated with DNA. The identification of SSG visualized by described standardized procedures was determined not only by cytochemical extraction tests but also by biological experiments. The latter demonstrated that the number of SSG in Yoshida sarcoma cells decreases after treatment of experimental animals with actinomycin D and therefore depends on the state of the nucleolar RNA synthesis.
在大鼠吉田肉瘤细胞涂片上研究了用于选择性显示核仁银染颗粒（SSG）以及同时显示SSG和核仁银染基质（SSM）的银染程序。这些程序的成功结果主要取决于硝酸银和甲醛的质量。然而，无论其来源和批次如何，这两种化学物质都可以很容易地标准化和稳定化。在标准化程序中（用于选择性显示SSG的一步法程序以及用于同时显示SSG和SSM的两步法程序），银显然与SSG和SSM的蛋白质酸性基团结合。SSG和SSM的蛋白质似乎不同，但都属于酸性非组蛋白。根据消化实验结果，也有可能SSG的酸性蛋白质与DNA相关。通过所述标准化程序可视化的SSG的鉴定不仅通过细胞化学提取试验确定，还通过生物学实验确定。后者表明，用放线菌素D处理实验动物后，吉田肉瘤细胞中SSG的数量减少，因此取决于核仁RNA合成的状态。
