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A simple method for incubation of tissue sections in immunohistochemistry.

作者信息

Ormanns W, Pfeifer U

出版信息

Histochemistry. 1981;72(2):315-9. doi: 10.1007/BF00517144.

DOI:10.1007/BF00517144
PMID:6168614
Abstract

The construction of a simple "incubation chamber" is presented by which a homogeneous incubation of tissue sections with calculable amounts of immunoreagents is guaranteed. The incubation of large area sections, and parallel incubations of 10 slides are possible. As an example of application a case of alpha-1-antitrypsin deficiency in the liver is demonstrated.

摘要

相似文献

1
A simple method for incubation of tissue sections in immunohistochemistry.
Histochemistry. 1981;72(2):315-9. doi: 10.1007/BF00517144.
2
An incubation chamber for the simultaneous, on-slide treatment of brain sections with different reagents.一种用于在载玻片上同时用不同试剂处理脑切片的孵育室。
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Detection of Pi Z phenotype individuals by alpha-1-antitrypsin (AAT) immunohistochemistry in paraffin-embedded liver tissue specimens.在石蜡包埋的肝组织标本中通过α-1抗胰蛋白酶（AAT）免疫组织化学检测Pi Z表型个体。
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[Immunohistochemical demonstration of alpha-1-antitrypsin in renal tubular epithelium of patients with and without alpha-1-antitrypsin deficiency].[α-1抗胰蛋白酶缺乏和不缺乏患者肾小管上皮细胞中α-1抗胰蛋白酶的免疫组织化学证明]
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引用本文的文献

1
Electron microscopic demonstration of calcitonin in human medullary carcinoma of thyroid by the immuno gold staining method.用免疫金染色法对人甲状腺髓样癌中降钙素进行电子显微镜显示。
Histochemistry. 1984;81(4):369-72. doi: 10.1007/BF00514331.
2
The use of monoclonal antibodies against thyroglobulin in immunoperoxidase techniques.免疫过氧化物酶技术中抗甲状腺球蛋白单克隆抗体的应用。
Histochemistry. 1983;78(2):177-80. doi: 10.1007/BF00489496.
3
Enhancement of immunohistochemical staining. Effects of serum volume and mixing.免疫组织化学染色的增强。血清体积和混合的影响。

本文引用的文献

1
INCUBATION CHAMBERS MADE OF PLASTIC.塑料制成的培养箱
Acta Histochem. 1963 Jun 30;15:389-91.
2
The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique.注入的辣根过氧化物酶在小鼠肾近端小管吸收的早期阶段：一种新技术的超微结构细胞化学研究
J Histochem Cytochem. 1966 Apr;14(4):291-302. doi: 10.1177/14.4.291.
3
Alpha 1 antitrypsinin the livers of patients with emphysema.
Science. 1972 Jan 7;175(4017):63-5. doi: 10.1126/science.175.4017.63.
Histochemistry. 1982;75(2):203-8. doi: 10.1007/BF00496011.
4
An alkaline-phosphatase staining method in avidin-biotin immunohistochemistry.抗生物素蛋白-生物素免疫组织化学中的碱性磷酸酶染色法
Histochemistry. 1985;82(5):421-4. doi: 10.1007/BF02450475.
4
Immunocytochemical diagnosis of alpha-1-antitrypsin deficiency.α-1-抗胰蛋白酶缺乏症的免疫细胞化学诊断
Am J Surg Pathol. 1978 Sep;2(3):275-81. doi: 10.1097/00000478-197809000-00004.