McMillan P J
Histochemistry. 1982;75(2):203-8. doi: 10.1007/BF00496011.
The present paper describes two simple procedures which enhance immunohistochemical staining. One is to cover the sections with a plastic film to keep the serum uniformly distributed and minimize its evaporation. Secondly, rocking of the slides has been introduced, causing the serum to flow back and forth under the plastic film. Using this system, it has been possible to test for the effect of mixing on an immunohistochemical reaction (the demonstration of calcitonin in thyroid C cells). It has been found that mixing definitely enhances the reaction during the first 8 h. No effect of serum volume was observed.
本文介绍了两种增强免疫组织化学染色的简单方法。一种是用塑料薄膜覆盖切片，以使血清均匀分布并尽量减少其蒸发。其次，引入了玻片摇晃，使血清在塑料薄膜下前后流动。使用该系统，可以测试混合对免疫组织化学反应的影响（甲状腺C细胞中降钙素的显示）。已发现混合在最初8小时内确实增强了反应。未观察到血清体积的影响。
