一种用于干扰素的小鼠白血病病毒产量降低半微量测定法。

A murine leukemia virus yield-reduction semi-microassay for interferon.

作者信息

Hsu L, Finke J H, Proffitt M R

出版信息

J Virol Methods. 1980;1(3):167-77. doi: 10.1016/0166-0934(80)90013-0.

Abstract

Interferon (IF) treatment of murine cells chronically producing infectious leukemia virus results in a decreased yield of extracellular virus. We have utilized this principle to develop a useful semi-microassay for IF. The assay incorporates the desirable features of both virus yield-reduction and plaque-reduction assays, yet has few of the undesirable features associated with many IF assays. Being a semi-micro assay, it is amenable to the screening of multiple samples for IF and is economical with respect to cell culture materials, reagents and amount of sample required for testing.

摘要

用干扰素（IF）处理长期产生感染性白血病病毒的鼠细胞，会导致细胞外病毒产量降低。我们利用这一原理开发了一种用于干扰素的实用半微量测定法。该测定法兼具病毒产量降低测定法和蚀斑减少测定法的优点，但几乎没有许多干扰素测定法所具有的缺点。作为一种半微量测定法，它适合对多个样本进行干扰素筛查，并且在细胞培养材料、试剂和测试所需样本量方面都很经济。