Aboud M, Kimchi R, Bakhanashvili M, Salzberg S
J Virol. 1981 Dec;40(3):830-8. doi: 10.1128/JVI.40.3.830-838.1981.
The effect of interferon on the biochemical properties and the maturation process of intracellular viral particles isolated from the cytoplasmic fraction of NIH/3T3 cells chronically infected with Moloney murine leukemia virus was investigated. By labeling these virions with either [35S]methionine or [3H]glucosamine, we demonstrated that they contain the same viral proteins and glycoproteins found in extracellular virions. Interferon treatment was found to reduce the rate of intracellular virus assembly. This effect was not a consequence of an interferon inhibition of viral RNA synthesis or its translation or a consequence of an interference with the posttranslational cleavage processing of viral precursor proteins, since all of these steps were not affected by interferon. However, the reduced rate of virus assembly could be attributed to the inhibition of viral protein glycosylation observed in interferon-treated cells. Nevertheless, despite this reduced rate, virus particles accumulated in interferon-treated cells. This accumulation was probably due to the strong inhibition of their final release from such cells.
研究了干扰素对从长期感染莫洛尼鼠白血病病毒的NIH/3T3细胞胞质部分分离出的细胞内病毒颗粒的生化特性和成熟过程的影响。通过用[35S]甲硫氨酸或[3H]葡糖胺标记这些病毒粒子，我们证明它们含有与细胞外病毒粒子中相同的病毒蛋白和糖蛋白。发现干扰素处理可降低细胞内病毒组装的速率。这种作用不是干扰素抑制病毒RNA合成或其翻译的结果，也不是干扰病毒前体蛋白翻译后切割加工的结果，因为所有这些步骤均不受干扰素影响。然而，病毒组装速率降低可归因于在干扰素处理的细胞中观察到的病毒蛋白糖基化受到抑制。尽管如此，尽管速率降低，但病毒颗粒仍在干扰素处理的细胞中积累。这种积累可能是由于其从这些细胞中的最终释放受到强烈抑制。
