Hokland P, Berg K
J Immunol. 1981 Oct;127(4):1585-8.
Polymorphonuclear leukocytes (PMN) from healthy volunteers were tested for ADCC activity against both erythrocyte and tumor targets with and without the addition of human leukocyte interferon (IFN). It was demonstrated that IFN within 30 to 60 min enhanced the reaction in a dose-dependent manner with minimal IFN doses ranging from 1 to 100 units. Formal proof that the augmenting agent was IFN was obtained by using pure IFN proteins in combination with both mock-IFN preparations, which showed no enhancing activity, and anti-IFN antisera, which inhibited the action of the completely purified IFN proteins. In the light of data demonstrating that the IFN effect was most pronounced when the IgG antibodies in the ADCC reaction were present in suboptimal amounts, it is hypothesized that IFN may play a special role in the early nonspecific immune response against non-self antigens.
对来自健康志愿者的多形核白细胞（PMN）进行了测试，检测其在添加和不添加人白细胞干扰素（IFN）的情况下对红细胞和肿瘤靶标的抗体依赖性细胞介导的细胞毒性（ADCC）活性。结果表明，在30至60分钟内，IFN以剂量依赖性方式增强反应，最小IFN剂量范围为1至100单位。通过将纯IFN蛋白与模拟IFN制剂（无增强活性）和抗IFN抗血清（抑制完全纯化的IFN蛋白的作用）联合使用，获得了增强剂为IFN的正式证据。鉴于数据表明，当ADCC反应中的IgG抗体以次优量存在时，IFN效应最为明显，因此推测IFN可能在针对非自身抗原的早期非特异性免疫反应中发挥特殊作用。
