Dick W, Cullmann W, Müller N, Adler K
J Clin Chem Clin Biochem. 1981 Jun;19(6):357-61. doi: 10.1515/cclm.1981.19.6.357.
A chromogenic assay for the determination of factor XII using the chromogenic substrate Chromozym PK was evaluated. The assay was linear in the range 10 U/l to more than 200 U/l. Using the assay, the normal range of factor XII (50 healthy volunteers at random) was 136 U/l +/- 27 U/l. Kallikrein-inhibiting concentrations of aprotinin did not influence factor XII. Comparison of the chromogenic with the clotting assay resulted in a correlation coefficient of r = 0.831 (p-value less than 0.001). In patients with deep vein thrombosis, factor XII level was found to be reduced to about 60% of normal activity.
对一种使用发色底物Chromozym PK测定因子XII的发色测定法进行了评估。该测定法在10 U/l至超过200 U/l的范围内呈线性。使用该测定法，因子XII的正常范围（随机选取50名健康志愿者）为136 U/l +/- 27 U/l。抑肽酶的激肽释放酶抑制浓度不影响因子XII。发色测定法与凝血测定法的比较得出相关系数r = 0.831（p值小于0.001）。在深静脉血栓形成患者中，发现因子XII水平降至正常活性的约60%。
