Gallimore M J, Jones D W, Wendel H P
Kent Haemophilia Centre, Kent and Canterbury Hospital, United Kingdom.
Thromb Res. 1999 Apr 15;94(2):103-9. doi: 10.1016/s0049-3848(98)00202-3.
Factor XII levels were determined in plasma samples from 75 patients before undergoing aortocoronary bypass grafting and from 40 healthy age-matched donors by using a microtitre plate adaptation of a new chromogenic peptide substrate assay kit for factor XII. The chromogenic peptide substrate assay values for factor XII correlated well with those obtained in clotting (r=0.90; y= 15.811+0.8236x) and immunochemical (r=0.88; y=17.90+0.817x) assays in the normal donor samples. Factor XII levels in the patients were significantly lower than those in the normal donors (83.3+/-23.2% versus 103.4+/-23.1: p=0.004), and nine patients (12%) had factor XII values below 50% compared with only one of the normal donors (2.5%). Factor XII levels and kallikrein-like activities (a measure of contact system activation) were followed before, during, and one day after cardiopulmonary bypass in 20 patients. Factor XII levels were significantly reduced, and kallikrein-like activities significantly elevated after 5 and 30 minutes cardiopulmonary bypass. One day after cardiopulmonary bypass both factor XII levels and kallikrein-like activities were significantly lower than preoperation values.
采用一种新的用于凝血因子XII的生色肽底物检测试剂盒的微量滴定板法，对75例接受主动脉冠状动脉搭桥术的患者及40名年龄匹配的健康供体的血浆样本进行凝血因子XII水平测定。在正常供体样本中，凝血因子XII的生色肽底物检测值与凝血检测（r = 0.90；y = 15.811 + 0.8236x）及免疫化学检测（r = 0.88；y = 17.90 + 0.817x）所得结果相关性良好。患者的凝血因子XII水平显著低于正常供体（83.3±23.2% 对103.4±23.1：p = 0.004），9例患者（12%）的凝血因子XII值低于50%，而正常供体中只有1例（2.5%）如此。对20例患者在体外循环前、中及术后一天监测凝血因子XII水平及激肽释放酶样活性（接触系统激活的一项指标）。体外循环5分钟和30分钟后，凝血因子XII水平显著降低，激肽释放酶样活性显著升高。体外循环术后一天，凝血因子XII水平及激肽释放酶样活性均显著低于术前值。
