通过银染法证明的人类衰老与核糖体基因活性之间的关系。

The relationship between aging and ribosomal gene activity in humans as evidenced by silver staining.

作者信息

Denton T E, Liem S L, Cheng K M, Barrett J V

出版信息

Mech Ageing Dev. 1981 Jan;15(1):1-7. doi: 10.1016/0047-6374(81)90002-6.

Abstract

Silver selectively stains nucleolar organizer regions (NORs) distributed on the D and G chromosomes of man. The number of NORs is fairly constant for a given individual but is highly variable within populations. When 2540 metaphases from lymphocytes were examined from 127 normal subjects, a mean NOR number of 7.3 was obtained with a mode of 7. No significant difference was found in mean NOR number between females and males. However, regression lines do show a decrease in NOR number with aging for both sexes but the rate of decline is more evident in females. Also, G chromosome NORs appear more stable than those in D chromosomes. Since silver--NOR staining is indicative of ribosomal gene activity, it is proposed that lymphocyte rDNA becomes less sensitive to phytohemagglutinin stimulation during aging. It is unique that this gene repression can be visualized with conventional staining and light microscopy.

摘要

银可选择性地对分布于人类D组和G组染色体上的核仁组织区（NORs）进行染色。对于特定个体而言，NORs的数量相当恒定，但在群体中则具有高度变异性。当对127名正常受试者的淋巴细胞中的2540个中期相进行检查时，平均NOR数量为7.3，众数为7。未发现女性和男性之间的平均NOR数量存在显著差异。然而，回归线确实显示出两性的NOR数量均随年龄增长而减少，但女性的下降速率更为明显。此外，G组染色体的NORs似乎比D组染色体的NORs更稳定。由于银染NORs表明核糖体基因的活性，因此有人提出，淋巴细胞的核糖体DNA（rDNA）在衰老过程中对植物血凝素刺激的敏感性降低。这种基因抑制能够通过传统染色和光学显微镜观察到，这一点很独特。