Pedrazzini E, Mamaev N, Slavutsky I
Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina.
Mol Pathol. 1998 Feb;51(1):39-42. doi: 10.1136/mp.51.1.39.
To present data obtained from human bone marrow preparations from healthy individual showing that the proportion of metaphases with silver stained nucleolar organiser region (AgNOR) chromosomes is associated with the age of the donor.
Bone marrow preparations from eight Russian and 10 Argentinian healthy individuals donating bone marrow for heterologous transplantation were studied by silver staining. The Russian bone marrow preparations were used directly, while the bone marrow specimens from Argentinian donors were incubated for 24 hours at 37 degrees C in F-10 medium with 15% fetal bovine serum. The slides were silver stained by the one step method of Howell and Black with slight modifications. Thirty metaphases with clearly defined D and G group chromosomes were scored for the numbers of AgNORs. All metaphases that were adjacent to silver stained interphase nuclei were analysed to assess the percentage of AgNOR positive mitoses. The Kruskal Wallis test and Kendall's rank correlation coefficient (rK) were used to assess the relation between age and the percentage of AgNOR positive cells.
The mean numbers (SE) of AgNORs per metaphase were 5.06 (0.17) and 5.56 (0.23) for the Russian and Argentinian groups, respectively, with no significant differences between the two groups. The common percentage of AgNOR positive cells decreased significantly as a function of age, with an rK = -0.57 (p < 0.0012).
The percentages of AgNOR negative metaphases in bone marrow from healthy individuals is strongly associated with age and this may be related to age related telomere loss.
展示从健康个体的人骨髓制备物中获得的数据，表明银染核仁组织区（AgNOR）染色体的中期比例与供体年龄相关。
对8名俄罗斯和10名阿根廷为异基因移植捐献骨髓的健康个体的骨髓制备物进行银染研究。俄罗斯的骨髓制备物直接使用，而阿根廷供体的骨髓标本在含15%胎牛血清的F-10培养基中于37℃孵育24小时。玻片采用Howell和Black的一步法进行银染，并稍作修改。对30个具有清晰界定的D组和G组染色体的中期进行AgNOR计数。分析所有与银染间期核相邻的中期，以评估AgNOR阳性有丝分裂的百分比。采用Kruskal Wallis检验和肯德尔等级相关系数（rK）评估年龄与AgNOR阳性细胞百分比之间的关系。
俄罗斯组和阿根廷组每个中期的AgNOR平均数（标准误）分别为5.06（0.17）和5.56（0.23），两组之间无显著差异。AgNOR阳性细胞的总体百分比随年龄显著下降，rK = -0.57（p < 0.0012）。
健康个体骨髓中AgNOR阴性中期的百分比与年龄密切相关，这可能与年龄相关的端粒丢失有关。
