Serra I, Alberghina M, Viola M, Giuffrida A M
Neurochem Res. 1981 May;6(5):595-605. doi: 10.1007/BF00964396.
The incorporation of [methyl-3H]thymidine into DNA, of [5-3H]uridine into RNA, and of [1-14C]leucine into proteins of cerebral hemispheres, cerebellum, and brainstem of guinea pigs after 80 hr of hypoxic treatment was measured. Both in vivo (intraventricular administration of labeled precursors) and in vitro (tissue slices incubation) experiments were performed. The labeling of macromolecules extracted from the various subcellular fractions of the above-mentioned brain regions was also determined. After hypoxic treatment the incorporation of the labeled precursors into DNA, RNA, and proteins was impaired to a different extent in the three brain regions and in the various subcellular fractions examined; DNA and RNA labeling in cerebellar mitochondria and protein labeling in microsomes of the three brain regions examined were particularly affected.
在对豚鼠进行80小时缺氧处理后，测定了[甲基-3H]胸腺嘧啶核苷掺入DNA、[5-3H]尿苷掺入RNA以及[1-14C]亮氨酸掺入大脑半球、小脑和脑干蛋白质中的情况。同时进行了体内实验（向脑室内注射标记前体）和体外实验（组织切片孵育）。还测定了从上述脑区不同亚细胞组分中提取的大分子的标记情况。缺氧处理后，在所检测的三个脑区以及不同亚细胞组分中，标记前体掺入DNA、RNA和蛋白质的过程在不同程度上受到损害；所检测的三个脑区的小脑线粒体中的DNA和RNA标记以及微粒体中的蛋白质标记受到的影响尤为明显。
