Mobbs B G, Johnson I E, Connolly J G
Prostate. 1980;1(1):37-51. doi: 10.1002/pros.2990010107.
With a protamine sulphate precipitation method, total and free cytosol AR was assayed in BPH and prostatic carcinoma tissue, in order to investigate possible differences in AR concentration that might relate to the histo-pathology of the tissue or to endocrine manipulation of the patients. Similar ranges of total cytosol AR concentrations were observed in BPH and untreated prostatic carcinoma, but the latter tended to have a higher proportion of apparently free sites. Moreover, the proportion of "free" sites in the untreated carcinoma tissue appeared to be related to the proportion of poorly differentiated carcinoma in the specimen. In patients whose endogenous androgen levels had been lowered by treatment, the proportion of free sites tended to be higher, but a considerable proportion of sites appeared to be still occupied. Carcinoma tissue from some estrogen-treated patients had high cytosol AR concentrations. It is suggested that, in some treated patients, androgens of adrenal origin may occupy some AR sites and that some carcinomas may contain a considerable concentration of nonfunctional AR.
采用硫酸鱼精蛋白沉淀法，对良性前列腺增生（BPH）组织和前列腺癌组织中的总胞浆雄激素受体（AR）和游离胞浆AR进行检测，以研究AR浓度可能存在的差异，这些差异可能与组织的组织病理学或患者的内分泌治疗有关。在BPH和未经治疗的前列腺癌中观察到总胞浆AR浓度范围相似，但后者明显游离位点的比例往往更高。此外，未经治疗的癌组织中“游离”位点的比例似乎与标本中低分化癌的比例有关。在内源性雄激素水平通过治疗降低的患者中，游离位点的比例往往更高，但仍有相当比例的位点似乎被占据。一些接受雌激素治疗患者的癌组织胞浆AR浓度较高。提示在一些接受治疗的患者中，肾上腺来源的雄激素可能占据一些AR位点，并且一些癌组织可能含有相当浓度的无功能AR。
