Clarke S M, Merchant D J
Prostate. 1980;1(1):87-94. doi: 10.1002/pros.2990010112.
The objective of this work was to design a simple and reproducible method of culturing human prostatic epithelial cells from attainable tissue. This type of system is essential for in vitro studies of prostatic cancer and benign prostatic hyperplasia (BPH). The most readily available source of prostatic tissue is the transurethral prostatic resection (TURP). This tissue can be obtained at surgery and processed immediately in the laboratory using standard cell culture techniques. By detailed sectioning and careful histologic examination of the tissue which represented the mirror image of each explant that was cultured, we found that tissue containing viable glandular structure yielded epithelial outgrowths. Pieces of tissue that did not yield epithelial outgrowth were composed only of fibromuscular stroma or contained acini which were damaged (usually cauterized). We concluded that this type of system can be used routinely to study prostatic disease on an individual basis.
这项工作的目的是设计一种简单且可重复的方法，用于从可获取的组织中培养人前列腺上皮细胞。这种系统对于前列腺癌和良性前列腺增生（BPH）的体外研究至关重要。前列腺组织最容易获取的来源是经尿道前列腺切除术（TURP）。这种组织可以在手术时获得，并在实验室中使用标准细胞培养技术立即进行处理。通过对所培养的每个外植体的镜像组织进行详细切片和仔细的组织学检查，我们发现含有存活腺结构的组织产生了上皮细胞生长。未产生上皮细胞生长的组织块仅由纤维肌性基质组成或含有受损（通常是烧灼）的腺泡。我们得出结论，这种系统可常规用于个体水平上的前列腺疾病研究。
