Tsugaya M, Ohtaguro K
Department of Urology, Nagoya City University Medical School.
Nihon Hinyokika Gakkai Zasshi. 1993 Nov;84(11):1948-53. doi: 10.5980/jpnjurol1989.84.1948.
Primary epithelial and fibroblast cells from benign hypertrophic prostate tissue were established. The prostate tissues were obtained by transurethral resection of the prostate or retropubic prostatectomy in patients with benign prostatic hypertrophy. Growth factors for cultured epithelial cells and fibroblasts were studied. The epithelial cells grew well in the WAJC-404 culture medium with insulin, epidermal growth factor and dexamethasone. Fibroblasts grew well in culture medium containing 10% fetal calf serum. The prostate tissue was stored at 4 degrees C for 7 days and no degenerative change in the stromal cells was seen during this period. Although epithelial cells did degenerate with the passage of time, epithelial cells cultured after storage for 4 days at 4 degrees C behaved similarly to those cultured immediately after being isolated. These primary cultures of epithelial cells and fibroblasts from hypertrophic prostate may be useful for various studies.
成功建立了来自良性前列腺增生组织的原代上皮细胞和成纤维细胞。前列腺组织通过经尿道前列腺切除术或耻骨后前列腺切除术从良性前列腺增生患者中获取。对培养上皮细胞和成纤维细胞的生长因子进行了研究。上皮细胞在含有胰岛素、表皮生长因子和地塞米松的WAJC - 404培养基中生长良好。成纤维细胞在含有10%胎牛血清的培养基中生长良好。前列腺组织在4℃下储存7天，在此期间未观察到基质细胞的退行性变化。尽管上皮细胞会随着时间推移而退化，但在4℃下储存4天后培养的上皮细胞与分离后立即培养的上皮细胞表现相似。这些来自增生性前列腺的上皮细胞和成纤维细胞的原代培养物可能对各种研究有用。
