Nozumi K, Sato R, Ito H, Maruoka M, Shimazaki J
Urol Int. 1981;36(2):79-87. doi: 10.1159/000280398.
The binding of dihydrotestosterone, R 1881 and R 5020 was examined in cytosols of normal, benign hypertrophic and cancerous tissues of the human prostates. Almost all samples obtained by open operation showed high affinity binding to these ligands. Dissociation constants of the binding to these ligands were approximately 10(-9) M irrespective of the pathological state of the prostates. Maximum binding sites for dihydrotestosterone seemed to be greater in normal tissues than in the pathological ones. However, maximum binding sites for R 1881 and R 5020 were not significantly different among the normal and pathological prostates examined in the present study. Moreover, some correlation was observed between the maximum binding sites for R 1881 and those for R 5020. The samples resected by TUR seemed to be inadequate for analyses of androgen binding.
在人前列腺的正常组织、良性增生组织和癌组织的胞液中检测了双氢睾酮、R 1881和R 5020的结合情况。通过开放手术获取的几乎所有样本都显示出对这些配体的高亲和力结合。无论前列腺的病理状态如何，与这些配体结合的解离常数约为10(-9)M。双氢睾酮的最大结合位点在正常组织中似乎比病理组织中更多。然而，在本研究中检测的正常和病理前列腺组织中，R 1881和R 5020的最大结合位点没有显著差异。此外，观察到R 1881的最大结合位点与R 5020的最大结合位点之间存在一定相关性。经尿道前列腺切除术（TUR）切除的样本似乎不适合用于雄激素结合分析。
